RNA and protein elongation rates in Saccharomyces cerevisiae
Copyright policy )RNA and protein elongation rates in Saccharomyces cerevisiae
The RNA elongation rate has been measured in yeast by the kinetics of appearance of radioactivity in the different molecular weight classes by the method first developed by Bremer and Yuan (1968). Despite the limitations caused by the breakdown of the 35s rRNA precursor, an estimate of 29 to 38 nucleotides/second at 30° has been obtained for the RNA elongation rate. The protein elongation rate has been calculated by the method of Maaloe and Kjeldgaard (1966) which consists of dividing the number of amino acids polymerized into protein per unit of time by the number of active ribosomes. This has given values of 7 to 9 amino acids/second at 30°. These numbers are of the same order as those found in Escherichia coli when corrected to 37°. Eucaryotic cells could thus have preserved part of the coupling found in bacteria between RNA and protein elongation rates.
Time Factors, Peptide Chain Elongation, Translational, Saccharomyces cerevisiae, Peptide Elongation Factors, Tritium, Fungal Proteins, Molecular Weight, Cytosine, Kinetics, RNA, Ribosomal, Isotope Labeling, Centrifugation, Density Gradient, RNA, Carbon Radioisotopes, Uracil, Mathematics
Time Factors, Peptide Chain Elongation, Translational, Saccharomyces cerevisiae, Peptide Elongation Factors, Tritium, Fungal Proteins, Molecular Weight, Cytosine, Kinetics, RNA, Ribosomal, Isotope Labeling, Centrifugation, Density Gradient, RNA, Carbon Radioisotopes, Uracil, Mathematics
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