Physical mapping of bacteriophage T4

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1981 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 184, pages 289-299 (issn: 0026-8925, eissn: 1432-1874,

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Authors: E, Niggemann; I, Green; H P, Meyer; W, Rüger;

doi: 10.1007/bf00272920

pmid: 6276698

Physical mapping of bacteriophage T4

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Abstract

The 134 positions of the cleavage sites of the restriction endonucleases XbaI, HaeII and EcoRI were determined for a cytosine-containing DNA of bacteriophage T4. This physical map was aligned with the genetic map. The T4 early regions were further identified by hybridization of RNA synthesized in vitro to the restriction fragments and two promoter regions were localized by filter binding tests and R-loop analysis.

Related Organizations

Ruhr University Bochum
Germany

Keywords

Genes, Viral, DNA, Viral, Operon, Escherichia coli, Chromosome Mapping, Nucleic Acid Hybridization, RNA, Viral, T-Phages, DNA Restriction Enzymes

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