Induction and in vitro culture of soybean crown gall tumors

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1983 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 2, pages 201-204 (issn: 0721-7714, eissn: 1432-203X,

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Authors: H C, Pedersen; J, Christiansen; R, Wyndaele;

doi: 10.1007/bf00270104

pmid: 24258052

Induction and in vitro culture of soybean crown gall tumors

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Abstract

Induction of crown galls on 4-6 week old soybean (Glycine max (L.) Merr.) plants cultured in growth chambers was obtained with Agrobacterium tumefaciens strains C58, T37 and ACH5. The crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. Transformation was tested by opine tests and by molecular hybridization of restricted cell DNA with T-DNA fragments. Protoplasts were isolated from suspension cultures. Transformed protoplasts regenerate cell walls, divide and form calli without an exogenous supply of hormones.

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Aarhus University
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