Mutations that overcome plasmid-mediated relaxation affect (p)ppGpp
Copyright policy )Mutations that overcome plasmid-mediated relaxation affect (p)ppGpp
A recombinant plasmid, pMY3, was constructed in this laboratory to express the amber suppressor allele, Su+7, of the tRNATrp gene from E. coli (Yarus, 1979a). This plasmid also relaxes control of the synthesis of all stable RNA species in its host cell after amino acid deprivation. Guanosine penta and tetra-phosphate (MSII and MSI) concentrations are reduced to about one-half the levels achieved by starving the host cells carrying the cloning vehicle (pMB9) alone.
- University of Colorado Boulder United States
- University of Colorado System United States
Recombination, Genetic, RNA, Bacterial, Phenotype, DNA Topoisomerases, Type I, Mutation, Escherichia coli, Guanosine Pentaphosphate, Guanine Nucleotides, Plasmids
Recombination, Genetic, RNA, Bacterial, Phenotype, DNA Topoisomerases, Type I, Mutation, Escherichia coli, Guanosine Pentaphosphate, Guanine Nucleotides, Plasmids
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