Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1992 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 11 (issn: 0721-7714, eissn: 1432-203X,

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Authors: P N, Angeloni; H Y, Rey; L A, Mroginski;

doi: 10.1007/bf00236268

pmid: 24213160

Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum

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Abstract

Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro.

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Instituto de Botánica del Nordeste

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