In this study we have examined the variation of mean power frequency (MPF) in the electromyogram (EMG) from the non-fatigued trapezius muscle, with different external hand loads, in different shoulder joint positions, different torques and different planes of movement. The study was limited to the functional range of movement in the shoulder joint. It was performed on 19 healthy subjects. Surface EMG was recorded, analysed by means of a computer programme and examined by regression analysis. Normalized MPF values were calculated by dividing the MPF by the individual average MPF for all positions and loads. The results indicated no major variation in normalized MPF. The largest systematic variation of normalized MPF, +/- 8%, was related to joint angle. We have concluded that the MPF value obtained initially can be used within the functional range of movement to calculate the relative decrease in MPF as a result of muscle fatigue, and that the decrease is significant if it exceeds 8% of the initial value.