
doi: 10.1007/bf00225025
pmid: 24196056
Chromosome samples were prepared on a plastic coverslip covered with a polyester membrane and were subjected to the C-banding treatment. The C-band pattern was obtained after Giemsa staining. The C-band positive regions of the Crepis chromosomes were identified, dissected out by irradiation with a micro-laser beam and recovered in Eppendorf tubes.
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