DNA “fingerprints” applied to paternity analysis in apples (Malus x domestica)

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1990 English Publisher:Springer Science and Business Media LLCJournal:Theoretical and Applied Genetics, volume 79, pages 763-768 (issn: 0040-5752, eissn: 1432-2242,

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Authors: H, Nybom; B A, Schaal;

doi: 10.1007/bf00224242

pmid: 24226736

DNA “fingerprints” applied to paternity analysis in apples (Malus x domestica)

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Abstract

Analysis of minisatellite DNA sequences, yielding so called DNA "fingerprints", has proven useful in paternity analysis for several different organisms. Here 64 apple seedlings, grown from seeds collected in an orchard with three cultivars, were analyzed using the M13 "fingerprint" probe. Paternity could be determined for 56 of the seedlings, 2 of which were derived through selfing. The analysis was facilitated by the occurrence of a multiallelic locus. The five different fragments determined by this locus migrated to similar positions, whether digesting the DNA with restriction enzymes TaqI or RsaI.

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