The effect of temperature on Cu accumulation and depuration in the tissues of Cerithidea cingulata was studied at 18 °C, 28 °C, and 38 °C. The LC50 value for 5 days was observed to be high at ambient temperature (28 °C) that at the high temperature (38 °C). The LC50 for 10 days at the low temperature (18 °C) was higher than at the ambient (28 °C). When exposed to sublethal concentrations for 48 hr, the tissue accumulation of Cu was found to be more at the high temperature than at the ambient and low temperature. The depuration process was faster at high temperatures, and the animals returned to normal condition after 36 hr. At the ambient and low temperature, Cu accumulation was slow and prolonged, and the animals resumed to normal condition after 48 and 72 hr, respectively. The gonad-digestive gland (GDG) complex was found to show greater changes in accumulation and depuration process when compared to foot and viscera.