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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao The Histochemical Jo...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
The Histochemical Journal
Article . 1995 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
The Histochemical Journal
Article . 1995 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
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Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions

Authors: P, Prentø;

Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions

Abstract

The paper takes issue with the use by glutaraldehyde suppliers of the term 'for electron microscopy', and the common practice of researchers giving insufficient or no data about the glutaraldehyde they use. Investigation of 11 commercial glutaraldehydes recommended for electron microscopy shows that only three or four of them are adequate for this purpose, using criteria set forth in papers dated between 1965 and 1989. The present paper reports that a check of purity can best be done by spectrophotometry. The 234/280 or 235/280 nm absorbance ratio is a precise indicator of the degree of polymerization, provided certain conditions stated in this paper are fulfilled. Some of the storage precautions taken by, or proposed by, suppliers are superfluous, and only mask the inadequate purification by the suppliers. A simple protocol for the storage of stock solutions is given. Alkaline glutaraldeyhyde is inherently very unstable, even in the refrigerator. Fixatives should, therefore, be stored in the freezer or should be freshly prepared.

Related Organizations
Keywords

Oxygen, Quality Control, Microscopy, Electron, Time Factors, Glutaral, Spectrophotometry, Temperature

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Top 10%
Top 10%
Average
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