Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid
Copyright policy )Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid
When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe-, Trp-, Tyr-) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.
- University of Bonn Germany
Carbon Isotopes, Base Sequence, Genetic Vectors, Molecular Sequence Data, Oxo-Acid-Lyases, Parabens, Recombinant Proteins, Klebsiella pneumoniae, Glucose, Bacterial Proteins, Isotope Labeling, Escherichia coli, Transformation, Bacterial, Anthranilate Synthase
Carbon Isotopes, Base Sequence, Genetic Vectors, Molecular Sequence Data, Oxo-Acid-Lyases, Parabens, Recombinant Proteins, Klebsiella pneumoniae, Glucose, Bacterial Proteins, Isotope Labeling, Escherichia coli, Transformation, Bacterial, Anthranilate Synthase
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