
doi: 10.1007/bf00134622
Cell-free extracts of Leuconostoc and Lactococcus species were tested for their alpha-acetolactate synthase and alpha-acetolactate decarboxylase activities. In Leuconostoc mesenteroides subsp. cremoris, Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc lactis, the Km of alpha-acetolactate synthase for pyruvate was close to 10 mM whereas it was 30 mM in Lactococcus lactis subsp. lactis biovar. diacetylactis. The Km of alpha-acetolactate decarboxylase for alpha-acetolactic acid was very low (0.3 mM) in Leuconostoc species in comparison to Lactococcus lactis subsp. lactis biovar. diacetylactis (60 mM). In the latter bacterium, alpha-acetolactate decarboxylase showed a sigmoidal dependance upon alpha-acetolactic acid and was activated by the three branched-chain amino acids: leucine, isoleucine and valine.
[SDV] Life Sciences [q-bio], products, lactis, co-metabolism, glucose;products;diacetylactis;lactis;citrate;co-metabolism;mesenteroides subsp cremoris, diacetylactis, citrate, glucose, mesenteroides subsp cremoris
[SDV] Life Sciences [q-bio], products, lactis, co-metabolism, glucose;products;diacetylactis;lactis;citrate;co-metabolism;mesenteroides subsp cremoris, diacetylactis, citrate, glucose, mesenteroides subsp cremoris
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