D-malic acid production from DL-malic acid by enantiospecific assimilation with Acinetobacter lwofii

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1993 English Publisher:Springer Science and Business Media LLCJournal:Biotechnology Letters, volume 15, pages 23-28 (issn: 0141-5492, eissn: 1573-6776,

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Authors: Masayo Miyama; Kiyoshi Nakayama;

doi: 10.1007/bf00131547

D-malic acid production from DL-malic acid by enantiospecific assimilation with Acinetobacter lwofii

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Abstract

Acinetobacter lwofii ATCC 9036 assimilated L-malic acid eantiospecifically and left D-malic acid when grown in a medium containing DL-malic acid. The optical purity of the D-malic acid isolated from the culture filtrate was 100%. When the organism was incubated at 26°C, 220 r.p.m. in a Erlenmeyer flask containing 100g/l of disodium maleate, L-malic acid was completely consumed during 7 days incubation and D-malic acid remained at the concentration of 35g/l.

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