Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1994 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell, Tissue and Organ Culture, volume 37, pages 205-208 (issn: 0167-6857, eissn: 1573-5044,

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Authors: Todd G. Guevin; Victor Micah; Edward G. Kirby;

doi: 10.1007/bf00043618

Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea

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Abstract

Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir [Abies balsamea (Mill.)] on media containing 10 μM cytokinin [6-(γ-γ-dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 μM) or on 4.5 μM BA in combination with 10 μM naphthyl-1-acetic acid. When ESMs were transferred to media containing 5–80 μM abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.

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Rutgers, The State University of New Jersey
United States

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