Multiple buds have been induced from cotyledonary nodes of cashew, Anacardium occidentale L. Maximum number of buds were initiated on Murashige & Skoog's (MS) medium supplemented with 117 mM sucrose, 14.6 mM maltose and 22.2 μM BA. The buds were harvested at each subculture after elongation on MS medium supplemented with 100 ml l-1 coconut water, 14.6 mM maltose, increased sucrose (146 mM) and decreased BA (4.4 μM). Excised microshoots were rooted in vitro on MS medium in the presence of 117 mM sucrose, 2.9 μM IAA and 4.9 μM IBA. Plantlets have been successfully transferred to soil and have been established in the field.