Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1990 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell, Tissue and Organ Culture, volume 20, pages 1-6 (issn: 0167-6857, eissn: 1573-5044,

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Authors: B. Campion; C. Alloni;

doi: 10.1007/bf00034750

Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules

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Abstract

Haploid plants have been obtained from unpollinated ovules of onion cultured in vitro as here reported for the first time. Callus was not formed at any step of the process and the young developing embryos sprouted by splitting the ovules. From a total of about 26000 cultured ovules, 13 embryos were obtained and 7 of them developed into plants. Cytological observation of root apexes showed that 3 embryos out of 7 regenerated haploid plants (8 chromosomes). The yield of embryo induction was quite low (0.28% the best treatment) so further experiments are in progress to increase it.

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