Structural and functional analysis of an Opaque-2-related gene from sorghum
Copyright policy )Structural and functional analysis of an Opaque-2-related gene from sorghum
The Opaque-2 (O2) gene from maize encodes a transcriptional activator of the b-ZIP class. We have isolated and characterized a gene from sorghum, related in sequence to the O2 gene from maize. A single copy of the gene is present in sorghum. Both genomic and cDNA sequences of the O2-related sorghum gene were determined. The sequence is highly homologous to maize O2 both in the promoter and in the coding region. The most closely related sequences contain the b-ZIP domain with only 11 amino acid substitutions in a total of 122 residues. In transient expression assays, the sorghum O2-related coding sequence, expressed from a CaMV 35S promoter, activates expression from the maize b-32 promoter as effectively as that obtained with the maize O2 sequence.
- Max Planck Institute for Plant Breeding Research Germany
- Max Planck Society Germany
Leucine Zippers, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Protein Sorting Signals, Genes, Plant, Poaceae, Transfection, Zea mays, DNA-Binding Proteins, Open Reading Frames, G-Box Binding Factors, Oligodeoxyribonucleotides, Sequence Homology, Nucleic Acid, Amino Acid Sequence, Promoter Regions, Genetic, Glucuronidase, Plant Proteins, Transcription Factors
Leucine Zippers, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Protein Sorting Signals, Genes, Plant, Poaceae, Transfection, Zea mays, DNA-Binding Proteins, Open Reading Frames, G-Box Binding Factors, Oligodeoxyribonucleotides, Sequence Homology, Nucleic Acid, Amino Acid Sequence, Promoter Regions, Genetic, Glucuronidase, Plant Proteins, Transcription Factors
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