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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Plant Growth Regulat...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Plant Growth Regulation
Article . 1996 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
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Sterilisation of explants and cultures with sodium dichloroisocyanurate

Authors: M. Parkinson; A. J. Sayegh; M. Prendergast;

Sterilisation of explants and cultures with sodium dichloroisocyanurate

Abstract

The effectiveness of Sodium dichloroisocyanurate as a disinfectant for micropropagated plants was assessed. Analysis of the microbial flora of micropropagated plants showed a wide range of bacteria with predominantly Pseudomonas, Xanthomonas and Actinomycetes. Sodium dichloroisocyanurate was highly stable both as preprepared tablets and as solutions maintained at room temperature. Sterilisation of a range of plants which were heavily contaminated with bacteria was examined. Phytotoxicity was generally low and restricted to old leaves and cut surfaces. Solutions of Sodium dichloroisocyanurate were more effective at high concentrations (5000 ppm) than a commercially available bleach for disinfection of shoot cultures. Sodium dichloroisocyanurate was also used at low concentrations (300 ppm) for longer periods (24 h–48 h) to disinfect shoot explants from the field, and was at least as effective for sterilisation as a combination of Mercuric Chloride and Calcium hypochlorite.

  • BIP!
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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    22
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
22
Top 10%
Top 10%
Average
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