Capsicum annuum L. produces a single flower and thus a single fruit per branching node. In contrast, Capsicum chinenseJacq. yields two or more flowers per node. If genes for multiple-flowers per node could be transferred from C. chinese to C. annuum, it might be possible to breed C. annuum varieties with a more concentrated fruit set and potentially higher yield. Using progeny from an interspecific cross between C. annuum cv. NM 6-4 and C. chinense CA4, it has been determined that a minimum of five independently segregating chromosomal regions control the difference in flowering behavior between these two accessions. One of the segments is located on a translocated chromosome. Epistatic interactions among independent chromosomal regions appear to play a major role in the determination of this trait.