Molecular diversity of three S-allele cDNAs associated with gametophytic self-incompatibility in Lycopersicon peruvianum
Copyright policy )Molecular diversity of three S-allele cDNAs associated with gametophytic self-incompatibility in Lycopersicon peruvianum
We isolated S allele-associated cDNA clones from each of the stylar cDNA libraries of Lycopersicon peruvianum of two different S genotypes (S12Sb and S13Sc) with S11Sa allele-associated cDNA (LPS11) as a probe. The longest cDNA clones, designated LPS12 and LPS13, which were 779 bp and 853 bp in length, contained open reading frames of 189 and 210 amino acids, respectively. The three S allele-associated cDNAs (LPS11, LPS12, and LPS13) did not cross-hybridize to each other under highly stringent condition by northern blot analysis. Their average identity to Nicotiana alata S-proteins so far was 49%. The fragments corresponding to LPS11 or LPS12 cosegregated with their respective S alleles in genetic crosses. From these results, we conclude that the three cloned cDNAs were derived from the three different S alleles of L. peruvianum.
- University of Tokyo Japan
Nicotiana, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Genetic Variation, Plants, Genes, Plant, Polymerase Chain Reaction, Open Reading Frames, Plants, Toxic, Amino Acid Sequence, Alleles, DNA Primers, Gene Library, Glycoproteins, Plant Proteins
Nicotiana, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Genetic Variation, Plants, Genes, Plant, Polymerase Chain Reaction, Open Reading Frames, Plants, Toxic, Amino Acid Sequence, Alleles, DNA Primers, Gene Library, Glycoproteins, Plant Proteins
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!