Analyses of Intracellular Signal Transduction Pathways in CNS Cells * Confocal and Electron Microscopic Trakcking of Internalized Neuropeptide/Receptor Complexes * Transfection Methods for Neurons in Primary Culture * Polyethylenimine: a Versatile Cationic Polymer for Plasmid-based Gene Delivery in the CNS * Trasfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK293 Cells * Neuronal Transfection Using Particle-Mediated Gene Transfer * Analysis of Gene Expression in Genetically Labeled Single Cells * Immunocytochemistry and In Situ Hybridization: Interest of their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons * In Situ Reverse Transcriptase PCR for Detection of mRNA in the CNS * Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity * Combined electrophysiological and morphological analysis of CNS neurons * Tract -Tracing Methods at the Light Microscopy Level * Tract-tracing Methods at the Ultrastructural Level * In vivo Analysis of Cell Proliferation and Apoptosis in the CNS * Confocal Imaging of Nerve Cells and their Connections * Confocal Imaging of Calcium Signaling in Cells from.