Anchoring of proteins to lactic acid bacteria
Copyright policy )Anchoring of proteins to lactic acid bacteria
The anchoring of proteins to the cell surface of lactic acid bacteria (LAB) using genetic techniques is an exciting and emerging research area that holds great promise for a wide variety of biotechnological applications. This paper reviews five different types of anchoring domains that have been explored for their efficiency in attaching hybrid proteins to the cell membrane or cell wall of LAB. The most exploited anchoring regions are those with the LPXTG box that bind the proteins in a covalent way to the cell wall. In recent years, two new modes of cell wall protein anchoring have been studied and these may provide new approaches in surface display. The important progress that is being made with cell surface display of chimaeric proteins in the areas of vaccine development and enzyme- or whole-cell immobilisation is highlighted.
- University of Groningen Netherlands
S-LAYER PROTEIN, GRAM-POSITIVE BACTERIA, VAGINAL IMMUNIZATION, Lactobacillus subsp, Gram-Positive Bacteria, Models, Biological, STAPHYLOCOCCAL SURFACE-PROTEINS, Bacterial Proteins, Cell Wall, NUCLEOTIDE-SEQUENCE, COMMENSAL BACTERIA, Lactic Acid, Staphylococcus carnosus, LACTOCOCCUS-LACTIS, Binding Sites, Cell Membrane, CELL-WALL ANCHOR, Lactococcus lactis, surface display, Staphylococcus xylosus, Streptococcus gordonii, STREPTOCOCCUS-GORDONII, MUCOSAL VACCINE DELIVERY, Biotechnology
S-LAYER PROTEIN, GRAM-POSITIVE BACTERIA, VAGINAL IMMUNIZATION, Lactobacillus subsp, Gram-Positive Bacteria, Models, Biological, STAPHYLOCOCCAL SURFACE-PROTEINS, Bacterial Proteins, Cell Wall, NUCLEOTIDE-SEQUENCE, COMMENSAL BACTERIA, Lactic Acid, Staphylococcus carnosus, LACTOCOCCUS-LACTIS, Binding Sites, Cell Membrane, CELL-WALL ANCHOR, Lactococcus lactis, surface display, Staphylococcus xylosus, Streptococcus gordonii, STREPTOCOCCUS-GORDONII, MUCOSAL VACCINE DELIVERY, Biotechnology
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