Over the last 25 years, stress echocardiography has become a widely used technique for diagnosis and risk stratification of patients with suspected or known coronary artery disease. However, relevant methodological limitations of conventional 2D stress echocardiography still exist. It remains difficult to acquire high-quality data and to analyze the images with reproducible results which may lead to reduced test accuracy and a lack of ability to detect regional myocardial ischemia.