In the recent digital era, biotechnology has also expanded the wings in the development of sophisticated intelligent devices. Points of care (POC) devices are one of the most recent and sophisticated digitalized applications of biotechnology and biochemistry. POC devices are a result of collaboration between biotechnology, biochemistry, electronics and marketing. POC devices employ technology for detection of various diseases and disorders without centralized, expensive, laboratory-specific equipment and tools. POC device is one of the best examples of lab-on-a-chip advancement, where only a small POC chip can efficiently perform the work in absence of a whole laboratory set-up. The best about POC devices is their easy, convenient, automated operational procedures, which made them user-friendly. Nowadays, where users prefer customized less time-consuming, portable and reliable methods for various purposes, POC devices are the most suitable technology to meet the demands. Nowadays, POC devices are serving various sectors of society, including healthcare, food safety and personal care. Major applications of POC devices are the test kits available in the market for detection of many clinical conditions. Pregnancy and diabetes test kits are the most popular and relatively cheaper older examples of POC technology. POC testing is a rapidly growing application of biotechnology, which offers new advancements and enormous growth possibilities for biotech professionals. Purpose of the present chapter is to focus on the attention of readers towards this relatively new, less popular but commercial application of biotechnology.