Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions

Frank M. Narendja; Georg Sauermann

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https://doi.org/10.1...arrow_drop_down

https://doi.org/10.1007/978-3-...

Part of book or chapter of book . 1996 . Peer-reviewed

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https://dx.doi.org/10.1007/978...

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Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Other literature type 01 Jan 1996 English Publisher:Birkhäuser Basel

Authors: Frank M. Narendja; Georg Sauermann;

doi: 10.1007/978-3-0348-7349-9_8

Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions

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Abstract

Methods are described for the preparation of poly(ADP-ribose) and for the preparation of biotinylated poly(ADP-ribose). Poly(ADP-ribose) is covalently modified to its biotinylated derivative by light-induced reaction of photobiotin with the polymer. Furthermore, conditions are given for the detection of poly(ADP-ribose) binding proteins in ligand-blotting experiments. The electrophoretically separated proteins are identified on blots using biotinylated poly(ADP-ribose) in a staining reaction. The results obtained with biotinylated poly(ADP-ribose) are comparable with those obtained with radioactive poly(ADP-ribose).

Related Organizations

University of Basel
Switzerland
Max Planck Institute of Biophysics
Germany
Max Planck Society
Germany

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