Genetic Transformation System
Genetic Transformation System
Lingzhi (Ganoderma lucidum) contains various bioactive constituents, such as polysaccharides and triterpenoids. The development of genetic transformation systems of Lingzhi is necessary for the characterization of the function of the target genes and the efficient production of bioactive compounds by genetically engineered strains. This chapter describes major genetic transformation methods for Lingzhi, namely, polyethylene glycol-mediated transformation (PMT), restriction enzyme-mediated integration (REMI), electroporation, and Agrobacterium tumefaciens-mediated transformation (ATMT). These transformation methods support the genetic engineering of Lingzhi to enhance polysaccharide and triterpenoid production. Gene silencing of Lingzhi through transformation is also described in this chapter. Additionally, recent advances in disrupting and deleting target genes using the CRISPR/Cas9 technology are discussed.
- Kunming University of Science and Technology China (People's Republic of)
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!