Enabling the precise deposition of various cells and biomaterials at pre defined position, 3D bioprinting techniques have emerged as a promising tool to recapitulate the actual microenvironment of native tissues/organs at in vitro system. Based on their working principle, the main techniques are mainly classified into extrusion-based, inkjet-based, and laser-assisted printing. In this chapter, their different features are discussed in terms of biofabrication strategies, resolution, cell viability, and applicable viscosities of biomaterials.