This chapter addresses the constitutionalization of biolaw in Colombia. The subject will be treated as follows. The second section will refer to the constitutionalization of law in general, its elements and the idea of constitutional supremacy attached to it. For its part, the third section will address the conceptualization of biolaw as a legal discipline. The relationship between biolaw and the constitutionalization of law, especially in the Colombian Constitutional Court’s case law, will be developed in the fourth section. The main conclusion is that biolaw, as a legal discipline in Colombia, has not made great progress at the legislative level but rather in the Constitutional Court through the emergence of some jurisprudence based upon international comparative law. This, according to the authors, has brought relevant benefits for Colombia in the protection of so-called fourth generation human rights or biorights.