descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 07 Oct 2012 English Publisher:Humana PressFunded by:SNSF | Software for Electron Mic...

Authors: Arheit, Marcel; Castaño-Díez, Daniel; Thierry, Raphaël; Gipson, Bryant R.; Zeng, Xiangyan; Stahlberg, Henning;

doi: 10.1007/978-1-62703-176-9_10

pmid: 23132061

Image Processing of 2D Crystal Images

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Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.

Related Organizations

University of Basel
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University System of Georgia
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Keywords

Crystallography, Fourier Analysis, Image Processing, Computer-Assisted, Membrane Proteins, Algorithms, Software

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