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Optimising Pichia pastoris Induction

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2012Publisher:Humana Press

Authors: Zharain Bawa; Richard A. J. Darby;

doi: 10.1007/978-1-61779-770-5_15

pmid: 22454123

Optimising Pichia pastoris Induction

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Abstract

A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.

Related Organizations

Aston University
United Kingdom
University of Oxford
United Kingdom
Oxford University Hospitals NHS Trust
United Kingdom
John Radcliffe Hospital
United Kingdom

Keywords

Bioreactors, Methanol, Fermentation, Pichia, Recombinant Proteins

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	5
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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average