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Immunoaffinity Chromatography

Name: Immunoaffinity Chromatography
Creator: Subramanian, Anuradha
Keywords: 570, chromatography, Biochemical and Biomolecular Engineering, 610, Indicators and Reagents, Immunoaffinity, Antigens, Antibodies, Chromatography, Affinity, Immunosorbent Techniques

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article , Other literature type 01 Jan 2000 United States Publisher:Humana PressJournal:Molecular Biotechnology, volume 20, pages 41-48 (eissn: 1073-6085,

Authors: Subramanian, Anuradha;

doi: 10.1007/978-1-60327-261-2_10 , 10.1385/mb:20:1:041 , 10.1385/1-59259-041-1:95

pmid: 11876298 , 10857089

Immunoaffinity Chromatography

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Abstract

Immunoaffinity chromatography is a process in which the binding affinity of an antigen to a parent antibody is utilized as a basis of separation. Owing to the customized avidity and specificity, monoclonal antibodies (Mabs) have become indispensable for both protein characterization and purification. The immunosorbent performance is dependent on the support matrix upon which the antibody is immobilized and on the activation chemistry used couple the antibody to the matrix. This report details, protocols to immobilize Mabs on commercially available supports, and a method to compute immunosorbent efficiency.

Country

United States

Related Organizations

University of Minnesota Morris
United States
University of Nebraska System
United States

Keywords

570, chromatography, Biochemical and Biomolecular Engineering, 610, Indicators and Reagents, Immunoaffinity, Antigens, Antibodies, Chromatography, Affinity, Immunosorbent Techniques

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	34
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average