
pmid: 17951808
PerlPrimer is a cross-platform application for the design of standard PCR, bisulfite PCR, real-time PCR, and sequencing primers. The program combines accurate primer-design algorithms with powerful interfaces to commonly used Internet databases, such as sequence retrieval from the Ensembl genome databases and the ability to BLAST search primer pairs. The use of PerlPrimer for designing primers is described, together with a synopsis of the primer search and primer-dimer algorithms.
User-Computer Interface, Computer Graphics, Sequence Analysis, DNA, Databases, Nucleic Acid, Polymerase Chain Reaction, Algorithms, Software, DNA Primers
User-Computer Interface, Computer Graphics, Sequence Analysis, DNA, Databases, Nucleic Acid, Polymerase Chain Reaction, Algorithms, Software, DNA Primers
| citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 18 | |
| popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Average | |
| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Top 10% | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Average |
