Urease is one of the most distinctive virulence factors of Proteus mirabilis pathogenesis. Urease activity correlates with many landmark side effects of P. mirabilis catheter-associated urinary tract infections, such as urolithiasis and bacteremia. Here we describe two simple and inexpensive colorimetric methods for quantifying urease activity in single species cultures as well as cocultures.

Related Organizations

California State University System
United States
California Polytechnic State University
United States

Keywords

Bacteriological Techniques, Virulence Factors, Calorimetry, Urease, Bacterial Proteins, Catheter-Related Infections, Urinary Tract Infections, Humans, Proteus Infections, Proteus mirabilis

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