Quantitative RNA Analysis Using RNA-Seq

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article , Other ORP type 01 Jan 2019 English Publisher:Springer New York

Authors: Myler, P. J.; McDonald, Jacqueline A.; Alcolea, Pedro J.; Sur, Aakash;

doi: 10.1007/978-1-4939-9210-2_4

pmid: 30980299

handle: 10261/181014

Quantitative RNA Analysis Using RNA-Seq

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Abstract

High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.

Related Organizations

Seattle Institute for Biomedical and Clinical Research
United States
University of Mary
United States

Keywords

Leishmania, Sequence Analysis, RNA, mRNA, High-Throughput Nucleotide Sequencing, Exons, RNA, Messenger, RNA-seq, Differential gene expression, RNA, Protozoan, Transcriptomes

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