Chromatin Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome. Here we describe the immunoprecipitation of Vibrio cholerae DNA bound to the histone-like nucleoid structuring protein (H-NS) tagged with the Flag epitope. The quality of the DNA obtained in this protocol is suitable for next-generation sequencing. The procedure described herein can be readily adapted to other bacteria and DNA-binding proteins.
- University of Alabama at Birmingham United States
- Morehouse School of Medicine United States
DNA-Binding Proteins, Chromatin Immunoprecipitation, Bacterial Proteins, High-Throughput Nucleotide Sequencing, Real-Time Polymerase Chain Reaction, Vibrio cholerae, Protein Binding
DNA-Binding Proteins, Chromatin Immunoprecipitation, Bacterial Proteins, High-Throughput Nucleotide Sequencing, Real-Time Polymerase Chain Reaction, Vibrio cholerae, Protein Binding
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