The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch's membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.

Related Organizations

Kiel University
Germany
University of Lübeck
Germany
University Medical Center of the Johannes Gutenberg University Mainz
Germany

Keywords

Tissue Culture Techniques, Organ Culture Techniques, Cell Survival, Choroid, Swine, Animals, Retinal Pigment Epithelium

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