Chromosome Painting of Mouse Chromosomes
Chromosome Painting of Mouse Chromosomes
Chromosome painting enables the visualization of chromosomes and has been used extensively in cytogenetics. Chromosome paint probes, which consist of a pooled composite of DNA-FISH probes, bind to nonrepetitive sequences for individual chromosomes [1, 2]. Here we describe the process of using chromosome paint to study the organization of chromosomes without fragmenting the nucleus. This method can be used to analyze chromosome position, and identify translocations and ploidy within the nucleus. The preservation of nuclear morphology is crucial in understanding interchromosomal interactions and dynamics in the nucleus during the cell cycle.
- University of California, San Francisco United States
Male, 1.1 Normal biological development and functioning, Cells, Chromosome paint, Chromosomes, Chromosome Painting, Cytogenetics, Mice, Underpinning research, Pregnancy, Genetics, Medicinal and biomolecular chemistry, Animals, DNA-FISH, Nuclear structure, Cells, Cultured, Nuclear organization, Cultured, Mammalian, Fluorescence in situ hybridization, Biological Sciences, Fibroblasts, Embryo, Mammalian, Embryo, Biochemistry and cell biology, Female, Biochemistry and Cell Biology, Other Chemical Sciences, Developmental Biology
Male, 1.1 Normal biological development and functioning, Cells, Chromosome paint, Chromosomes, Chromosome Painting, Cytogenetics, Mice, Underpinning research, Pregnancy, Genetics, Medicinal and biomolecular chemistry, Animals, DNA-FISH, Nuclear structure, Cells, Cultured, Nuclear organization, Cultured, Mammalian, Fluorescence in situ hybridization, Biological Sciences, Fibroblasts, Embryo, Mammalian, Embryo, Biochemistry and cell biology, Female, Biochemistry and Cell Biology, Other Chemical Sciences, Developmental Biology
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