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Genome Editing of Silkworms

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2017Publisher:Springer New York

Authors: Hideki Sezutsu; Takuya Tsubota;

doi: 10.1007/978-1-4939-7128-2_17

pmid: 28643261

Genome Editing of Silkworms

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Abstract

Silkworm is a lepidopteran insect that has been used as a model for a wide variety of biological studies. The microinjection technique is available and it is possible to cause transgenesis as well as target gene disruption via the genome editing technique. TALEN-mediated knock-out is especially effective in this species. We also succeeded in the precise and efficient integration of a donor vector using the Precise Integration into Target Chromosome (PITCh) method. Here, we describe protocols for ZFN, TALEN, and CRISPR/Cas9-mediated genome editing as well as the PITCh technique in the silkworm. We consider that all of these techniques can contribute to the further promotion of various biological studies in the silkworm and other insect species.

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Keywords

Gene Editing, Microinjections, Transcription Activator-Like Effector Nucleases, Genome, Insect, Animals, Gene Knock-In Techniques, CRISPR-Cas Systems, Bombyx, Zinc Finger Nucleases

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	15
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%