
In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.
Male, Meiosis, Chromosomes; Drosophila; Immunostaining; Live analysis; Male meiosis; Meiotic spindle; Spermatogenesis; Molecular Biology; Genetics, Cytological Techniques, Testis, Animals, Drosophila, Spindle Apparatus, Chromosomes, Insect
Male, Meiosis, Chromosomes; Drosophila; Immunostaining; Live analysis; Male meiosis; Meiotic spindle; Spermatogenesis; Molecular Biology; Genetics, Cytological Techniques, Testis, Animals, Drosophila, Spindle Apparatus, Chromosomes, Insect
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