Comet Assay in Cancer Chemoprevention
Comet Assay in Cancer Chemoprevention
The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.
- McMaster University Canada
- Sapienza University of Rome Italy
- Juravinski Cancer Centre Canada
Cell Death, Staining and Labeling, Neoplasms, DNA Breaks, Double-Stranded, Comet Assay, DNA Breaks, Single-Stranded, Comet assay; DNA damage; DNA repair; Double-strand breaks (DSB); Genotoxic stress; Single-cell gel electrophoresis (SCGE); Single-strand breaks (SSB); Cell Death; Comet Assay; DNA Breaks, Double-Stranded; DNA Breaks, Single-Stranded; Molecular Imaging; Neoplasms; Staining and Labeling; Chemoprevention; Molecular Biology; Genetics, Chemoprevention, Molecular Imaging
Cell Death, Staining and Labeling, Neoplasms, DNA Breaks, Double-Stranded, Comet Assay, DNA Breaks, Single-Stranded, Comet assay; DNA damage; DNA repair; Double-strand breaks (DSB); Genotoxic stress; Single-cell gel electrophoresis (SCGE); Single-strand breaks (SSB); Cell Death; Comet Assay; DNA Breaks, Double-Stranded; DNA Breaks, Single-Stranded; Molecular Imaging; Neoplasms; Staining and Labeling; Chemoprevention; Molecular Biology; Genetics, Chemoprevention, Molecular Imaging
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).8 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!