High-Throughput Phenomics
High-Throughput Phenomics
Standard protocols are available in order to apply Phenotype MicroArray (PM) technology to characterize different groups of microorganisms. Nevertheless, there is the need to pay attention to several crucial steps in order to obtain high-quality and reproducible data from PM, such as the choice of the Dye mix, the type and concentration of the carbon source in metabolic experiments, the use of a buffered medium. A systematic research of auxotrophies in strains to be tested should be carefully evaluated before starting with PM experiments. Detailed protocols to obtain defined and reproducible phenotypic profiles for bacteria and yeasts are shown. Moreover, the innovative software opm R packages and DuctApe suite for the analysis of kinetic data produced by PM and panphenome description are reported.
- New York University Italy
- University of Florence Italy
- European Bioinformatics Institute United Kingdom
- European Molecular Biology Laboratory Germany
Bacteria, Nitrogen, Fungi, Phosphorus, Microbial Sensitivity Tests, Hydrogen-Ion Concentration, Microarray Analysis, Carbon, Culture Media, High-Throughput Screening Assays, Phenotype, Nephelometry and Turbidimetry, Metabolic Networks and Pathways, Software, Sulfur, Fluorescent Dyes
Bacteria, Nitrogen, Fungi, Phosphorus, Microbial Sensitivity Tests, Hydrogen-Ion Concentration, Microarray Analysis, Carbon, Culture Media, High-Throughput Screening Assays, Phenotype, Nephelometry and Turbidimetry, Metabolic Networks and Pathways, Software, Sulfur, Fluorescent Dyes
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