Cloning and Characterization of a Murine Platelet-Activating Factor Receptor Gene
Cloning and Characterization of a Murine Platelet-Activating Factor Receptor Gene
A murine gene encoding a platelet-activating factor receptor (PAFR) was cloned. The gene was mapped to a distal region of chromosome 4 both by fluorescence in situ hybridization and by molecular linkage analysis. Northern blot analysis showed a high expression of the PAFR message in peritoneal macrophages. When C3H/HeN macrophages were treated with bacterial lipopolysaccharide (LPS) or synthetic lipid A, the PAFR gene expression was induced. Bacterial LPS, but not lipid A, induced the level of PAFR mRNA in LPS-unresponsive C3H/HeJ macrophages. These induction patterns were parallel to those of tumor necrosis factor-alpha mRNA. Thus, the PAFR in macrophages plays important roles in LPS-induced pathologies.
- University of Tokyo Japan
- Life Science Institute Japan
- Japan Tobacco (United States) United States
Lipopolysaccharides, Mice, Inbred C3H, Guinea Pigs, Chromosome Mapping, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Receptors, G-Protein-Coupled, Mice, Inbred C57BL, Muridae, Mice, Lipid A, Gene Expression Regulation, Genes, Organ Specificity, Macrophages, Peritoneal, Animals, Female, RNA, Messenger, Cloning, Molecular, Gene Library
Lipopolysaccharides, Mice, Inbred C3H, Guinea Pigs, Chromosome Mapping, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Receptors, G-Protein-Coupled, Mice, Inbred C57BL, Muridae, Mice, Lipid A, Gene Expression Regulation, Genes, Organ Specificity, Macrophages, Peritoneal, Animals, Female, RNA, Messenger, Cloning, Molecular, Gene Library
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