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Multiplexing Autoradiography

Name: Multiplexing Autoradiography
Creator: David Y, Lewis
Keywords: Radioisotopes, Fluorine Radioisotopes, Positron-Emission Tomography, Autoradiography, Radiopharmaceuticals, Fluorescent Dyes, 3. Good health

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 26 Nov 2023Publisher:Springer US

Authors: David Y, Lewis;

doi: 10.1007/978-1-0716-3499-8_24

pmid: 38006510

Multiplexing Autoradiography

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Abstract

Autoradiography, the direct imaging of radioactive distribution in tissue sections, is a powerful technique that has several key advantages for the validation of PET radiotracers. Using autoradiography, we can localize radiotracer uptake to neighbours of cells, and when multiplexed with additional radiotracers, fluorescent probes, or in situ tissue analysis, autoradiography can help to characterize the mechanism of radiotracer uptake and assess functional heterogeneity in tissue. In this chapter, the author outlines the basic ex vivo autoradiography protocol and shows how it can be multiplexed using dual radionuclides 18F and 14C. They also highlight where autoradiography can be combined with other technologies to provide synergistic information for interrogating spatial biology.

Related Organizations

Cancer Research UK Scotland Institute
United Kingdom
University of Glasgow
United Kingdom

Keywords

Radioisotopes, Fluorine Radioisotopes, Positron-Emission Tomography, Autoradiography, Radiopharmaceuticals, Fluorescent Dyes

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