Purification and Immunophenotypic Characterization of Human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B Cells
Purification and Immunophenotypic Characterization of Human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ B Cells
Regulatory B cells (Bregs) have immunosuppressive capacity, primarily via the production of IL-10. IL-10 expression and immunosuppression have been described in a number of human B cell subsets, two of which include the CD19+CD24hiCD38hi and CD19+CD24hiCD27+ populations. In this chapter, we describe how to identify and isolate these subsets from peripheral blood B cells via flow cytometry. We also explain how to expand Bregs in culture and how to identify them based on intracellular expression of IL-10.
- University College London United Kingdom
- UNIVERSITY COLLEGE LONDON, Bartlett School of Planning United Kingdom
B-Lymphocytes, B-Lymphocytes, Regulatory, Immune regulation, Antigens, CD19, B-Lymphocyte Subsets, Cell Culture Techniques, Regulatory B cells, CD24 Antigen, Autoimmunity, Cell Separation, Flow Cytometry, Immunophenotyping, Interleukin-10, Phenotyping, Antigens, CD, IL-10, Humans, Flow cytometry
B-Lymphocytes, B-Lymphocytes, Regulatory, Immune regulation, Antigens, CD19, B-Lymphocyte Subsets, Cell Culture Techniques, Regulatory B cells, CD24 Antigen, Autoimmunity, Cell Separation, Flow Cytometry, Immunophenotyping, Interleukin-10, Phenotyping, Antigens, CD, IL-10, Humans, Flow cytometry
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