With the development of new imaging modalities, techniques,and radiotracers, it is easy to get caught up in the excitement of the equipment and the pictures. Yet the value in imaging lies primarily in the benefit it brings to human lives. That potential value is determined first by studies in animals. This paper will focus on the care and handling of rodents as they make up over 90% of the animal research done. However,many of the concepts we will be discussing could be applied to any species. Whatever species you work with, take time to learn about them and their specific needs; it will pay compounding dividends.