Identification of Target Genes Regulated by PAX3 and PAX3–FKHR in Embryogenesis and Alveolar Rhabdomyosarcoma
Copyright policy )Identification of Target Genes Regulated by PAX3 and PAX3–FKHR in Embryogenesis and Alveolar Rhabdomyosarcoma
PAX3 is a transcription factor important for neural, muscle, and facial development in vertebrates. To identify genes regulated by PAX3, we used a cyclic amplification and selection of targets (CASTing) strategy to isolate cis-regulatory elements bound by PAX3. CASTing libraries were constructed with mouse DNA fragments bound by mouse PAX3, and human genomic DNA fragments bound by human PAX3 and the fusion protein PAX3-FKHR. Approximately 1000 clones were sequenced from each of these three libraries. Numerous putative targets of PAX3 and PAX3-FKHR were identified and six genes, Itm2A, Fath, FLT1, TGFA, BVES, and EN2, were examined closely. The genomic DNA fragments near these genes contain PAX3 binding sites and confer PAX3-dependent regulation. The expression levels of these genes correlate with the PAX3 expression levels in mouse embryos or with PAX3-FKHR expression levels in rhabdomyosarcoma cell lines, and indicate they may be part of the PAX3 regulatory circuitry during embryogenesis and tumor formation.
- National Institute of Health Pakistan
- National Institute on Deafness and Other Communication Disorders United States
- University of Pennsylvania United States
- Michigan State University United States
- National Institutes of Health United States
Extracellular Matrix Proteins, Nonmuscle Myosin Type IIB, Myosin Heavy Chains, Forkhead Box Protein O1, Molecular Sequence Data, Gene Expression Regulation, Developmental, Membrane Proteins, Muscle Proteins, Forkhead Transcription Factors, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Mice, Gene Targeting, Animals, Humans, Paired Box Transcription Factors, Cell Adhesion Molecules, PAX3 Transcription Factor, Rhabdomyosarcoma, Alveolar, Gene Library
Extracellular Matrix Proteins, Nonmuscle Myosin Type IIB, Myosin Heavy Chains, Forkhead Box Protein O1, Molecular Sequence Data, Gene Expression Regulation, Developmental, Membrane Proteins, Muscle Proteins, Forkhead Transcription Factors, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Mice, Gene Targeting, Animals, Humans, Paired Box Transcription Factors, Cell Adhesion Molecules, PAX3 Transcription Factor, Rhabdomyosarcoma, Alveolar, Gene Library
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).64 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!