
pmid: 12547215
EAAC1 is a widely expressed protein which transports anionic amino acids in a Na(+)-dependent fashion. Rat erythrocytes have generally been thought to be impermeant to anionic amino acids. Utilizing immunoelectron microscopy, we have demonstrated the presence of EAAC1-immunoreactive protein within rat erythrocytes. Immunoblotting revealed the presence of an approximately 60-kDa protein, consistent with EAAC1, in erythrocyte membranes. Specificity was confirmed by peptide competition. In conclusion, EAAC1 is expressed in rat erythrocytes.
Rats, Sprague-Dawley, Glutamate Plasma Membrane Transport Proteins, Erythrocytes, Excitatory Amino Acid Transporter 3, Symporters, Amino Acid Transport System X-AG, Animals, Immunohistochemistry, Rats
Rats, Sprague-Dawley, Glutamate Plasma Membrane Transport Proteins, Erythrocytes, Excitatory Amino Acid Transporter 3, Symporters, Amino Acid Transport System X-AG, Animals, Immunohistochemistry, Rats
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