Negative Regulation of YY1 Transcription Factor on the Dynamin I Gene Promoter
Copyright policy )Negative Regulation of YY1 Transcription Factor on the Dynamin I Gene Promoter
Dynamin I is highly expressed in brain and plays a critical role in clathrin-mediated endocytosis and synaptic vesicle recycling. To elucidate the molecular mechanism by which expression of dynamin I is tissue-specifically regulated, we previously cloned and characterized the promoter of the mouse dynamin I gene and suggested that there is a negative regulatory element in this promoter region. In the present study, we showed that YY1 binds to this negative regulatory element located at -111 to -107 by using the EMSA and supershift analyses. Cotransfection experiment using an YY1 expression vector revealed that YY1 exerts a repressive role on the dynamin I gene promoter activity. These results demonstrate that transcription factor YY1 negatively regulates dynamin I expression via binding to the negative regulatory element.
- UPRRP College of Natural Sciences Puerto Rico
- Korea Research Institute of Bioscience and Biotechnology Korea (Republic of)
Dynamins, Binding Sites, Base Sequence, DNA, Transfection, Binding, Competitive, Cell Line, GTP Phosphohydrolases, DNA-Binding Proteins, Mice, Gene Expression Regulation, Animals, Erythroid-Specific DNA-Binding Factors, Promoter Regions, Genetic, Dynamin I, YY1 Transcription Factor, Transcription Factors
Dynamins, Binding Sites, Base Sequence, DNA, Transfection, Binding, Competitive, Cell Line, GTP Phosphohydrolases, DNA-Binding Proteins, Mice, Gene Expression Regulation, Animals, Erythroid-Specific DNA-Binding Factors, Promoter Regions, Genetic, Dynamin I, YY1 Transcription Factor, Transcription Factors
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