Interaction between Smad Anchor for Receptor Activation and Smad3 Is Not Essential for TGF-β/Smad3-Mediated Signaling

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2001 Japan English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 281, pages 1,100-1,105 (issn: 0006-291X,

Copyright policy )

Authors: D. Goto; H. Nakajima; Y. Mori; K. Kurasawa; N. Kitamura; I. Iwamoto;

doi: 10.1006/bbrc.2001.4489

pmid: 11243848

Interaction between Smad Anchor for Receptor Activation and Smad3 Is Not Essential for TGF-β/Smad3-Mediated Signaling

- Summary
- Subjects
- Metrics

Abstract

Regulation of subcellular localization of Smad proteins is supposed to be critical for the effective initiation and maintenance of TGF-beta signaling. Recently, Smad anchor for receptor activation (SARA) has been identified as a Smad2 binding protein. SARA regulates the subcellular localization of Smad2 and is required for TGF-beta/Smad2-mediated signaling. In this study, we determined whether the interaction between SARA and Smad3 is essential for TGF-beta/Smad3-mediated signaling. We found that a mutant Smad3 (Smad3NS) that lacked the binding to SARA was phosphorylated by TGF-beta type I receptor at the similar level to that in wild-type Smad3 (Smad3WT). Smad3NS also formed complexes with Smad4 and translocalized into the nucleus. Moreover, Smad3NS and Smad3WT equally enhanced TGF-beta-induced transcription. Therefore, these findings indicate that, in contrast to SARA/Smad2 interaction, SARA/Smad3 interaction is not essential for TGF-beta/Smad3-mediated signaling.

Country

Japan

Related Organizations

Institute of Science Tokyo
Japan
Chiba University
Japan

Keywords

Cell Nucleus, Transcriptional Activation, Endosomal Sorting Complexes Required for Transport, Macromolecular Substances, Active Transport, Cell Nucleus, Receptor, Transforming Growth Factor-beta Type I, Protein Serine-Threonine Kinases, 540, Phosphoproteins, Transfection, DNA-Binding Proteins, COS Cells, Mutation, Trans-Activators, Animals, Smad3 Protein, Phosphorylation, Carrier Proteins, Activin Receptors, Type I, Receptors, Transforming Growth Factor beta, Signal Transduction

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	38
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%