Cytotoxic Activity of Recombinant bFGF–rViscumin Fusion Proteins
Copyright policy )Cytotoxic Activity of Recombinant bFGF–rViscumin Fusion Proteins
A fusion protein (bFGF-rMLA), containing the mitogen basic fibroblast growth factor (bFGF) and the cytotoxic component of rViscumin (recombinant mistletoe lectin), the enzymatic A-chain (rMLA), was expressed in Escherichia coli, purified, and functionally characterized. bFGF-rMLA is cytotoxic for mouse B16 melanoma cells expressing the FGF receptor with an IC(50) value of approximately 1 nM. rMLA shows no significant effect on the viability of the B16 cells up to a concentration of 141 nM. Additionally, bFGF-rMLA was associated with the rViscumin B-chain (rMLB) in an in vitro folding procedure. The IC(50) value of bFGF-rMLA/rMLB to B16 cells in the presence of lactose-to block rMLB lectin activity-was 134 pM. Thus, it was possible to enhance the efficacy of a rViscumin A-chain mitotoxin through addition of rMLB. We conclude that rViscumin fusion proteins may be generally applicable for the receptor-specific inactivation of target cells and point out their potential in drug development.
Protein Folding, Cell Death, Dose-Response Relationship, Drug, Recombinant Fusion Proteins, Melanoma, Experimental, Oligonucleotides, Protein Structure, Tertiary, Ribosome Inactivating Proteins, Type 2, Inhibitory Concentration 50, Kinetics, Mice, Lectins, Escherichia coli, Animals, Electrophoresis, Polyacrylamide Gel, Fibroblast Growth Factor 2, Plant Preparations, Cloning, Molecular, Plant Proteins, Plasmids
Protein Folding, Cell Death, Dose-Response Relationship, Drug, Recombinant Fusion Proteins, Melanoma, Experimental, Oligonucleotides, Protein Structure, Tertiary, Ribosome Inactivating Proteins, Type 2, Inhibitory Concentration 50, Kinetics, Mice, Lectins, Escherichia coli, Animals, Electrophoresis, Polyacrylamide Gel, Fibroblast Growth Factor 2, Plant Preparations, Cloning, Molecular, Plant Proteins, Plasmids
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).5 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!